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Nat Neurosci 3 : 12821290

Brains were rapidly extracted and immersed in chilled cutting solution (250 mM sucrose, 26 mM NaHCO 3 , 10 mM D(+)-glucose, 4 mM MgCl 2 , 3 mM myo-inositol, 2.5 mM KCl, 2 mM sodium pyruvate, 1.25 mM NaH 2 PO 4 , 0.5 mM ascorbic acid, 0.1 mM CaCl 2 , and 1 mM kynurenic acid, pH 7.4, continuously oxygenated with 95% O 2 and 5% CO 2 )

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Data extraction was performed independently by two researchers using a predesigned data extraction form